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J Biol Chem. 2017 Sep 8;292(36):14851-14866. doi: 10.1074/jbc.M117.798397. Epub 2017 Jul 24.

Chromatin-remodeling SWI/SNF complex regulates coenzyme Q6 synthesis and a metabolic shift to respiration in yeast.

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From the Department of Chemistry and Biochemistry.
the Molecular Biology Institute, and.
the Department of Molecular Cell and Developmental Biology, UCLA, Los Angeles, California 90095.
From the Department of Chemistry and Biochemistry,
the Molecular Biology Institute, and


Despite its relatively streamlined genome, there are many important examples of regulated RNA splicing in Saccharomyces cerevisiae Here, we report a role for the chromatin remodeler SWI/SNF in respiration, partially via the regulation of splicing. We find that a nutrient-dependent decrease in Snf2 leads to an increase in splicing of the PTC7 transcript. The spliced PTC7 transcript encodes a mitochondrial phosphatase regulator of biosynthesis of coenzyme Q6 (ubiquinone or CoQ6) and a mitochondrial redox-active lipid essential for electron and proton transport in respiration. Increased splicing of PTC7 increases CoQ6 levels. The increase in PTC7 splicing occurs at least in part due to down-regulation of ribosomal protein gene expression, leading to the redistribution of spliceosomes from this abundant class of intron-containing RNAs to otherwise poorly spliced transcripts. In contrast, a protein encoded by the nonspliced isoform of PTC7 represses CoQ6 biosynthesis. Taken together, these findings uncover a link between Snf2 expression and the splicing of PTC7 and establish a previously unknown role for the SWI/SNF complex in the transition of yeast cells from fermentative to respiratory modes of metabolism.


Saccharomyces cerevisiae; alternative splicing; chromatin remodeling; coenzyme Q; gene expression; lipid metabolism; metabolic regulation; mitochondrial metabolism; phosphorylation

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