Format

Send to

Choose Destination
J Biol Chem. 2017 Sep 8;292(36):14851-14866. doi: 10.1074/jbc.M117.798397. Epub 2017 Jul 24.

Chromatin-remodeling SWI/SNF complex regulates coenzyme Q6 synthesis and a metabolic shift to respiration in yeast.

Author information

1
From the Department of Chemistry and Biochemistry.
2
the Molecular Biology Institute, and.
3
the Department of Molecular Cell and Developmental Biology, UCLA, Los Angeles, California 90095.
4
From the Department of Chemistry and Biochemistry, cathy@chem.ucla.edu.
5
the Molecular Biology Institute, and tljohnson@ucla.edu.

Abstract

Despite its relatively streamlined genome, there are many important examples of regulated RNA splicing in Saccharomyces cerevisiae Here, we report a role for the chromatin remodeler SWI/SNF in respiration, partially via the regulation of splicing. We find that a nutrient-dependent decrease in Snf2 leads to an increase in splicing of the PTC7 transcript. The spliced PTC7 transcript encodes a mitochondrial phosphatase regulator of biosynthesis of coenzyme Q6 (ubiquinone or CoQ6) and a mitochondrial redox-active lipid essential for electron and proton transport in respiration. Increased splicing of PTC7 increases CoQ6 levels. The increase in PTC7 splicing occurs at least in part due to down-regulation of ribosomal protein gene expression, leading to the redistribution of spliceosomes from this abundant class of intron-containing RNAs to otherwise poorly spliced transcripts. In contrast, a protein encoded by the nonspliced isoform of PTC7 represses CoQ6 biosynthesis. Taken together, these findings uncover a link between Snf2 expression and the splicing of PTC7 and establish a previously unknown role for the SWI/SNF complex in the transition of yeast cells from fermentative to respiratory modes of metabolism.

KEYWORDS:

Saccharomyces cerevisiae; alternative splicing; chromatin remodeling; coenzyme Q; gene expression; lipid metabolism; metabolic regulation; mitochondrial metabolism; phosphorylation

PMID:
28739803
PMCID:
PMC5592666
DOI:
10.1074/jbc.M117.798397
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center