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J Biol Chem. 2017 Sep 8;292(36):14940-14962. doi: 10.1074/jbc.M117.797944. Epub 2017 Jul 24.

Sustained O-GlcNAcylation reprograms mitochondrial function to regulate energy metabolism.

Author information

1
From the Departments of Biochemistry and Molecular Biology.
2
Pharmacology, Toxicology and Therapeutics.
3
Biostatistics.
4
Molecular and Integrative Physiology.
5
Pathology and Laboratory Medicine, and.
6
the Department of Biological Chemistry, The Johns Hopkins University of Medicine, Baltimore, Maryland 21205.
7
Neurology, University of Kansas Medical Center and.
8
University of Kansas Alzheimer's Disease Center, Kansas City, Kansas 64108 and.
9
From the Departments of Biochemistry and Molecular Biology, cslawson@kumc.edu.

Abstract

Dysfunctional mitochondria and generation of reactive oxygen species (ROS) promote chronic diseases, which have spurred interest in the molecular mechanisms underlying these conditions. Previously, we have demonstrated that disruption of post-translational modification of proteins with β-linked N-acetylglucosamine (O-GlcNAcylation) via overexpression of the O-GlcNAc-regulating enzymes O-GlcNAc transferase (OGT) or O-GlcNAcase (OGA) impairs mitochondrial function. Here, we report that sustained alterations in O-GlcNAcylation either by pharmacological or genetic manipulation also alter metabolic function. Sustained O-GlcNAc elevation in SH-SY5Y neuroblastoma cells increased OGA expression and reduced cellular respiration and ROS generation. Cells with elevated O-GlcNAc levels had elongated mitochondria and increased mitochondrial membrane potential, and RNA-sequencing analysis indicated transcriptome reprogramming and down-regulation of the NRF2-mediated antioxidant response. Sustained O-GlcNAcylation in mouse brain and liver validated the metabolic phenotypes observed in the cells, and OGT knockdown in the liver elevated ROS levels, impaired respiration, and increased the NRF2 antioxidant response. Moreover, elevated O-GlcNAc levels promoted weight loss and lowered respiration in mice and skewed the mice toward carbohydrate-dependent metabolism as determined by indirect calorimetry. In summary, sustained elevation in O-GlcNAcylation coupled with increased OGA expression reprograms energy metabolism, a finding that has potential implications for the etiology, development, and management of metabolic diseases.

KEYWORDS:

O-GlcNAcase; O-GlcNAcylation; OGT; bioenergetics; mitochondria; nuclear factor 2 (erythroid-derived 2-like factor) (NFE2L2) (Nrf2); reactive oxygen species (ROS)

PMID:
28739801
PMCID:
PMC5592672
DOI:
10.1074/jbc.M117.797944
[Indexed for MEDLINE]
Free PMC Article

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