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Blood. 2017 Sep 7;130(10):1243-1246. doi: 10.1182/blood-2017-04-777987. Epub 2017 Jul 24.

Immunoassay for human serum erythroferrone.

Author information

1
Department of Medicine and.
2
Department of Pathology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, CA.
3
Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
4
UCSF Benioff Children's Hospital Oakland, Oakland, CA.
5
UC Riverside School of Medicine, Riverside, CA.
6
University of Victoria, Victoria, BC, Canada; and.
7
Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM U1220, Institut National de la Recherche Agronomique U1416, Ecole Nationale Vétérinaire de Toulouse, Université Paul Sabatier, Toulouse, France.

Abstract

Erythroferrone (ERFE) is a glycoprotein hormone secreted by erythroblasts in response to stimulation by erythropoietin (EPO). We previously demonstrated that ERFE messenger RNA expression and serum protein concentration increase in mice subjected to hemorrhage or EPO therapy, that ERFE acts on hepatocytes to suppress hepcidin, and that the resulting decrease in hepcidin augments iron delivery for intensified erythropoiesis. We also showed that ERFE contributes to pathological hepcidin suppression and iron overload in mice with nontransfused β-thalassemia. We now report the development and technical validation of a rabbit monoclonal antibody-based sandwich immunoassay for human ERFE. We use this assay to show that blood loss or EPO administration increases serum ERFE concentrations in humans, and that patients with both nontransfused and transfused β-thalassemia have very high serum ERFE levels, which decrease after blood transfusion. The assay should be useful for human studies of normal and disordered erythropoiesis and its effect on iron homeostasis.

PMID:
28739636
PMCID:
PMC5606005
DOI:
10.1182/blood-2017-04-777987
[Indexed for MEDLINE]
Free PMC Article

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