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Monoclon Antib Immunodiagn Immunother. 2017 Oct;36(5):224-230. doi: 10.1089/mab.2017.0027. Epub 2017 Jul 24.

PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry.

Author information

1
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
3
3 North Lab , Sapporo, Japan .
4
4 Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
5
5 Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
6
6 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .

Abstract

Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, dog PDPN, and bovine PDPN have been established, anticat PDPN (cPDPN) mAbs have not been developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs. One of the clones, PMab-52 (IgM, kappa), detected cPDPN specifically in flow cytometry and Western blot analysis. PMab-52 is also useful for detecting feline squamous cell carcinoma cells in immunohistochemical analysis. PMab-52 is expected to be useful for investigating the function of cPDPN in feline carcinomas.

KEYWORDS:

PDPN; cat podoplanin; immunohistochemistry; monoclonal antibody

PMID:
28737447
DOI:
10.1089/mab.2017.0027
[Indexed for MEDLINE]

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