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Biotechnol Rep (Amst). 2017 Jul 6;15:92-100. doi: 10.1016/j.btre.2017.06.007. eCollection 2017 Sep.

Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake.

Author information

1
College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
2
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental, Science and Engineering, Tongji University, Shanghai, China.
3
Biological and Cultural Diversity Research Center, Arba Minch, Ethiopia.
4
Centre for Technology in Water and Wastewater, School of Civil and Environmental, Engineering, University of Technology Sydney, Sydney, NWS 2007, Australia.

Abstract

Textile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decolorization. Based on 16S rRNA gene sequence analysis, strain CH12 was identified as Bacillus sp. Decolorization efficiencies were significantly enhanced with carbon (≥98%) and organic nitrogen (∼100%) supplements. Complete decolorization was also observed under anoxic and anaerobic conditions, and at the temperature of 30 °C and the pH of 10. However, the azo dye decolorization efficiency of strain CH12 was significantly reduced when NaNO3 (1-8%) was supplemented or under aerobic culturing condition (≤6%), indicating that RR239 was less preferred electron acceptor. Overall, strain CH12 can be a promising candidate for decolorization applications due to its potential to effectively decolorize higher RR239 concentrations (50-250 mg/L) and six additional dyes.

KEYWORDS:

Alkaline lake; Bacillus sp.; Biodecolorization; Reactive Red 329; Strain CH12

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