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Biomed Opt Express. 2017 Mar 9;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 1.

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).

Author information

1
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
2
Contributed equally.
3
Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201 USA.
4
Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.

Abstract

Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging in vivo.

KEYWORDS:

(170.2520) Fluorescence microscopy; (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6960) Tomography

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