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Biomed Opt Express. 2017 Mar 9;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 1.

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).

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Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Contributed equally.
Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201 USA.
Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.


Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging in vivo.


(170.2520) Fluorescence microscopy; (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6960) Tomography

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