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Chemosphere. 2017 Oct;185:852-859. doi: 10.1016/j.chemosphere.2017.07.103. Epub 2017 Jul 19.

The influence of heavy metals on toxicogenetic damage in a Brazilian tropical river.

Author information

1
Departamento de Ciências Biológicas, Universidade Federal do Piauí, Campus Ministro Petrônio Portela, Av. Universitária, CEP - 64.049-550, Brazil.
2
Departamento de Ciências Biológicas, Universidade Federal do Piauí, Campus Senador Helvídio Nunes de Barros, Av. Cícero Duarte, Picos, Piauí, CEP - 64600-000, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Campus Ministro Petrônio Portela, Bairro Ininga, Teresina, Piauí, CEP - 64049-550, Brazil. Electronic address: jmarcelo@ufpi.edu.br.
3
Departamento de Ciências Biológicas, Universidade Federal do Piauí, Campus Senador Helvídio Nunes de Barros, Av. Cícero Duarte, Picos, Piauí, CEP - 64600-000, Brazil.
4
Departamento de Ciências Biológicas, Universidade Federal do Piauí, Campus Senador Helvídio Nunes de Barros, Av. Cícero Duarte, Picos, Piauí, CEP - 64600-000, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Campus Ministro Petrônio Portela, Bairro Ininga, Teresina, Piauí, CEP - 64049-550, Brazil.
5
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Campus Ministro Petrônio Portela, Bairro Ininga, Teresina, Piauí, CEP - 64049-550, Brazil.
6
Departamento de Ciências Biológicas, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná, CEP - 87020-900, Brazil.

Abstract

Anthropogenic activities in tropical rivers favor the eutrophication process, which causes increased concentration of heavy metals. The presence and bioaccumulation of metals are directly related to the presence of genotoxic damage in aquatic organisms. Thus, we evaluated the presence of heavy metals (Fe, Zn, Cr, Cu and Al) and performed toxicogenetic tests in surface (S) and bottom (B) of water samples of the Poti river (Piaui/Brazil). Cytotoxicity and genotoxicity tests were performed in Allium cepa, and micronucleus (MN) and comet assay were performed in Oreochromis niloticus. The chemical analysis showed concentrations above the limit for Cu, Cr, Fe and Al according to Brazilian laws, characterizing anthropogenic disturbance in this aquatic environment. Toxicogenetic analysis presented significant cytotoxic, mutagenic and genotoxic effects in different exposure times and water layers (S and B), especially alterations in mitotic spindle defects, MN formations, nuclear bud and DNA strand breaks. Correlations between Fe and cytotoxicity, and Al and mutagenicity were statistically significant and point out to the participation of heavy metals in genotoxic damage. Therefore, Poti river water samples presented toxicogenetic effects on all bioindicators analyzed, which are most likely related to heavy metals pollution.

KEYWORDS:

Cytotoxicity; Ecotoxicology; Environmental mutagenesis; Genotoxicity; Tropical river

[Indexed for MEDLINE]

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