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Immunobiology. 2017 Nov;222(11):979-988. doi: 10.1016/j.imbio.2017.07.002. Epub 2017 Jul 11.

Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication.

Author information

1
Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea.
2
Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.
3
Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
4
College of Medicine and Medical Research Institute, Chungbuk National University, Chungdae-ro 1, Seowon-Ku, Cheongju, Republic of Korea.
5
Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea. Electronic address: oshin@korea.ac.kr.

Abstract

Schlafen (SLFN) proteins have been suggested to play important functions in cell proliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhanced viral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-β signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

KEYWORDS:

Anti-viral; Influenza; Interferon; SLFN14; VZV

PMID:
28734654
PMCID:
PMC5990420
DOI:
10.1016/j.imbio.2017.07.002
[Indexed for MEDLINE]
Free PMC Article

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