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Bioresour Technol. 2018 Jan;248(Pt B):104-112. doi: 10.1016/j.biortech.2017.07.061. Epub 2017 Jul 14.

High-throughput sequencing analysis of bacterial community spatiotemporal distribution in response to clogging in vertical flow constructed wetlands.

Author information

1
College of Water Conservancy and Hydroelectric Power, Hohai University, Nanjing 210098, PR China. Electronic address: huaguofen2005@126.com.
2
College of Water Conservancy and Hydroelectric Power, Hohai University, Nanjing 210098, PR China.
3
College of Harbour, Coastal and Offshore Engineering, Hohai University, Nanjing 210098, PR China.

Abstract

The aim of this study was to characterize bacterial communities in vertical flow constructed wetlands (VFCWs) using Illumina high-throughput sequencing. The bacterial communities developed lower richness and diversity in response to clogging. Bacterial diversity did not overtly decrease with depth. A variety of bacterial phyla were found in VFCWs' bacterial communities, including Bacteroidetes, Actinobacteria and Acidobacteria, among which Proteobacteria was dominant. At the genus level, a spatiotemporal variation was illustrated in the diversity and structure of bacterial communities. Clustering analysis of bacterial composition in the operational taxonomic units (OTUs) at the phylum and genus levels had a consistent trend, namely, that bacterial communities were more similar at similar column depths.

KEYWORDS:

Bacteria; Clogging; Cluster analysis; High-throughput sequencing; Vertical flow constructed wetland

PMID:
28734588
DOI:
10.1016/j.biortech.2017.07.061
[Indexed for MEDLINE]

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