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Biophys J. 2017 Sep 19;113(6):1321-1330. doi: 10.1016/j.bpj.2017.06.050. Epub 2017 Jul 19.

Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.

Author information

1
MRC Human Immunology UnitWeatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
2
Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
3
CNRS UMR 7213, Laboratoire de Biophotonique et Pharmacologie, University of Strasbourg, Illkirch Cedex, France.
4
MRC Human Immunology UnitWeatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom; Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. Electronic address: christian.eggeling@rdm.ox.ac.uk.

Abstract

The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity.

PMID:
28734477
PMCID:
PMC5607142
DOI:
10.1016/j.bpj.2017.06.050
[Indexed for MEDLINE]
Free PMC Article

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