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Cell Mol Life Sci. 2018 Jan;75(2):193-208. doi: 10.1007/s00018-017-2595-9. Epub 2017 Jul 21.

Current knowledge on exosome biogenesis and release.

Author information

1
Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, 0379, Oslo, Norway.
2
Centre for Cancer Biomedicine, University of Oslo, 0379, Oslo, Norway.
3
Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, 0379, Oslo, Norway. Alicia.Martinez.Llorente@rr-research.no.
4
Centre for Cancer Biomedicine, University of Oslo, 0379, Oslo, Norway. Alicia.Martinez.Llorente@rr-research.no.

Abstract

Exosomes are nanosized membrane vesicles released by fusion of an organelle of the endocytic pathway, the multivesicular body, with the plasma membrane. This process was discovered more than 30 years ago, and during these years, exosomes have gone from being considered as cellular waste disposal to mediate a novel mechanism of cell-to-cell communication. The exponential interest in exosomes experienced during recent years is due to their important roles in health and disease and to their potential clinical application in therapy and diagnosis. However, important aspects of the biology of exosomes remain unknown. To explore the use of exosomes in the clinic, it is essential that the basic molecular mechanisms behind the transport and function of these vesicles are better understood. We have here summarized what is presently known about how exosomes are formed and released by cells. Moreover, other cellular processes related to exosome biogenesis and release, such as autophagy and lysosomal exocytosis are presented. Finally, methodological aspects related to exosome release studies are discussed.

KEYWORDS:

Autophagy; Endosomes; Exosomes; Extracellular vesicles; Lysosomes; MVB biogenesis; MVBs; Microvesicles; Release; Secretion

PMID:
28733901
PMCID:
PMC5756260
DOI:
10.1007/s00018-017-2595-9
[Indexed for MEDLINE]
Free PMC Article

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