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Plant Mol Biol. 2017 Aug;94(6):669-685. doi: 10.1007/s11103-017-0635-3. Epub 2017 Jul 21.

Metabolic profiles of flooding-tolerant mechanism in early-stage soybean responding to initial stress.

Author information

1
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan.
2
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, 305-8518, Japan.
3
College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China.
4
Graduate School of Life and Food Sciences, Niigata University, Niigata, 950-2181, Japan.
5
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan. komatsu.setsuko.fu@u.tsukuba.ac.jp.
6
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, 305-8518, Japan. komatsu.setsuko.fu@u.tsukuba.ac.jp.

Abstract

Metabolomic analysis of flooding-tolerant mutant and abscisic acid-treated soybeans suggests that accumulated fructose might play a role in initial flooding tolerance through regulation of hexokinase and phosphofructokinase. Soybean is sensitive to flooding stress, which markedly reduces plant growth. To explore the mechanism underlying initial-flooding tolerance in soybean, mass spectrometry-based metabolomic analysis was performed using flooding-tolerant mutant and abscisic-acid treated soybeans. Among the commonly-identified metabolites in both flooding-tolerant materials, metabolites involved in carbohydrate and organic acid displayed same profile at initial-flooding stress. Sugar metabolism was highlighted in both flooding-tolerant materials with the decreased and increased accumulation of sucrose and fructose, respectively, compared to flooded soybeans. Gene expression of hexokinase 1 was upregulated in flooded soybean; however, it was downregulated in both flooding-tolerant materials. Metabolites involved in carbohydrate/organic acid and proteins related to glycolysis/tricarboxylic acid cycle were integrated. Increased protein abundance of phosphofructokinase was identified in both flooding-tolerant materials, which was in agreement with its enzyme activity. Furthermore, sugar metabolism was pointed out as the tolerant-responsive process at initial-flooding stress with the integration of metabolomics, proteomics, and transcriptomics. Moreover, application of fructose declined the increased fresh weight of plant induced by flooding stress. These results suggest that fructose might be the critical metabolite through regulation of hexokinase and phosphofructokinase to confer initial-flooding stress in soybean.

KEYWORDS:

Abscisic acid; Flooding stress; Metabolic profile; Mutant; Soybean

PMID:
28733872
DOI:
10.1007/s11103-017-0635-3
[Indexed for MEDLINE]

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