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Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.prot091959. doi: 10.1101/pdb.prot091959.

DNA Preparation from Schizosaccharomyces pombe.

Author information

1
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518 roguev@gmail.com.
2
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518.

Abstract

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

PMID:
28733411
DOI:
10.1101/pdb.prot091959

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