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Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.prot091942. doi: 10.1101/pdb.prot091942.

Transformation of Schizosaccharomyces pombe in a 96-Well Format.

Author information

1
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518 roguev@gmail.com.
2
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518.

Abstract

This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.

PMID:
28733397
DOI:
10.1101/pdb.prot091942

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