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Genome. 2017 Oct;60(10):815-824. doi: 10.1139/gen-2017-0029. Epub 2017 Jul 21.

Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae).

Author information

1
a Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia.
2
b Novosibirsk State University, 630090 Novosibirsk, Russia.
3
c Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.

Abstract

Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.

KEYWORDS:

A chromosomes; B chromosomes; FISH on three-dimensionally preserved nuclei; FISH sur des noyaux ayant préservé leur structure tridimensionnelle; architecture tridimensionnelle du noyau; centromeres; centromères; chromosomes A; chromosomes B; confocal microscopy; microscopie confocale; spermatocytes; three-dimensional architecture of the nucleus

PMID:
28732174
DOI:
10.1139/gen-2017-0029
[Indexed for MEDLINE]

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