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Methods Mol Biol. 2017;1632:303-324. doi: 10.1007/978-1-4939-7138-1_20.

Preparation of a Conditional RNA Switch.

Author information

1
RNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.
2
RNA Structure and Design Section, RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.
3
Leidos Biomedical Research Inc., Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.
4
Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, MD, USA.
5
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
6
The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
7
RNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA. shapirbr@mail.nih.gov.

Abstract

RNA has gained great interest for use in biomedical and therapeutic applications. This is due in part to RNA's ability to perform multiple functions, including the regulation of endogenously expressed genes. However, the ability of RNA based drugs to distinguish target diseased cells from healthy tissue remains challenging. Here we present methods for the production of a recently developed conditional RNA switch that releases a Dicer substrate RNA in response to interaction with a specific RNA biomarker.

KEYWORDS:

Conditional RNA; Nanomedicine; Nanotechnology; RNA; RNA switch; RNase H; Toehold

PMID:
28730448
DOI:
10.1007/978-1-4939-7138-1_20
[Indexed for MEDLINE]

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