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Methods Mol Biol. 2017;1632:269-283. doi: 10.1007/978-1-4939-7138-1_18.

Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.

Author information

1
Department of Biological Sciences, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, NC, 28223, USA.
2
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, 9201 University Blvd., Charlotte, NC, 28223, USA.
3
HIV Dynamics and Replication Program, NCI, Frederick, MD, 21702, USA.
4
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, 9201 University Blvd., Charlotte, NC, 28223, USA. kafonin@uncc.edu.
5
The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC, 28223, USA. kafonin@uncc.edu.
6
Center for Cancer Research, National Cancer Institute, Frederick, Maryland, 21702, USA. kafonin@uncc.edu.

Abstract

Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.

KEYWORDS:

Dicer substrate RNA; HIV-1; Hybrids reassociation; RNA interference; RNA nanotechnology; RNA–DNA hybrids

PMID:
28730446
DOI:
10.1007/978-1-4939-7138-1_18
[Indexed for MEDLINE]

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