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Anal Bioanal Chem. 2017 Sep;409(22):5209-5216. doi: 10.1007/s00216-017-0491-7. Epub 2017 Jul 20.

Aptamer-based fluorescence-quenching lateral flow strip for rapid detection of mercury (II) ion in water samples.

Author information

1
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, 510632, China.
2
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, 510632, China. tyjaq7926@163.com.
3
Institute of Biotranslational Medicine, Jinan University, Guangzhou, 510632, China. tyjaq7926@163.com.

Abstract

Divalent mercury ion (Hg2+) is one of the most common and stable forms of mercury pollution. In this study, a skillfully designed lateral flow strip (LFS) was developed for sensitive detection of Hg2+ in river water samples. Aptamer, a specific oligonucleotide probe, was used to selectively identify and target Hg2+ instead of antibody in traditional immunechromatographic strips; and the fluorescence-quenching system was used to generate positive and low background florescence signals in the competitive-likely LFS. The linear detection range of the LFS for Hg2+ was 0.13 ng mL-1 to 4 ng mL-1 and the limit of detection (LOD) was 0.13 ng mL-1. This test provided results in 15 min and demonstrated high specificity. For detection of Hg2+ in river water, the results were consistent with inductively coupled plasma-mass spectrometry measurements. The aptamer-based fluorescence-quenching LFS was shown to provide a reliable, accurate method for rapid detection of mercury contamination. Graphical Abstract The principle of the aptamer-based fluorescence-quenching LFS.

KEYWORDS:

Aptamer; Fluorescence-quenching; Lateral flow strip; Low-background; Mercury (II) ions

PMID:
28730311
DOI:
10.1007/s00216-017-0491-7

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