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Anal Bioanal Chem. 2017 Sep;409(23):5593-5602. doi: 10.1007/s00216-017-0497-1. Epub 2017 Jul 20.

A simple and rapid infrared-assisted self enzymolysis extraction method for total flavonoid aglycones extraction from Scutellariae Radix and mechanism exploration.

Author information

1
School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.
2
China State Institute of Pharmaceutical Industry, 285 Gebaini Road, Shanghai, 201203, China.
3
Microdyne Technologies Inc., Shanghai, 200235, China.
4
School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China. yuyj@fudan.edu.cn.
5
China State Institute of Pharmaceutical Industry, 285 Gebaini Road, Shanghai, 201203, China. guiliangc@hotmail.com.
6
School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China. glduan@shmu.edu.cn.

Abstract

A new, simple, and fast infrared-assisted self enzymolysis extraction (IRASEE) approach for the extraction of total flavonoid aglycones (TFA) mainly including baicalein, wogonin, and oroxylin A from Scutellariae Radix is presented to enhance extraction yield. Extraction enzymolysis temperature, enzymolysis liquid-to-solid ratio, enzymolysis pH, enzymolysis time and infrared power, the factors affecting IRASEE procedure, were investigated in a newly designed, temperature-controlled infrared-assisted extraction (TC-IRAE) system to acquire the optimum analysis conditions. The results illustrated that IRASEE possessed great advantages in terms of efficiency and time compared with other conventional extraction techniques. Furthermore, the mechanism of IRASEE was preliminarily explored by observing the microscopic change of the samples surface structures, studying the main chemical compositions change of the samples before and after extraction and investigating the kinetics and thermodynamics at three temperature levels during the IRASEE process. These findings revealed that IRASEE can destroy the surface microstructures to accelerate the mass transfer and reduce the activation energy to intensify the chemical process. This integrative study presents a simple, rapid, efficient, and environmental IRASEE method for TFA extraction which has promising prospects for other similar herbal medicines. Graphical Abstract ᅟ.

KEYWORDS:

Infrared-assisted self enzymolysis extraction; Kinetics and thermodynamics; Scutellariae Radix; Temperature-controlled infrared-assisted extraction system

PMID:
28730309
DOI:
10.1007/s00216-017-0497-1
[Indexed for MEDLINE]

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