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Sci Rep. 2017 Jul 20;7(1):6016. doi: 10.1038/s41598-017-06233-9.

Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium.

Author information

1
BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland. heidi.m.hongisto@staff.uta.fi.
2
Department of Ophthalmology, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
3
BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
4
Stem Cells and Eye Research Laboratory, Department of Ophthalmology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
5
Singapore Eye Research Institute and Duke-NUS School of Medicine, Singapore, Singapore.
6
Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Oslo, Norway.
7
Tampere University Hospital Eye Center, University of Tampere, Tampere, Finland.

Abstract

Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ). 1041 common proteins were present in both hESC-RPE cells and native hRPE with majority of the proteins similarly regulated. The hESC-RPE proteome reflected that of normal hRPE with a large number of metabolic, mitochondrial, cytoskeletal, and transport proteins expressed. No signs of increased stress, apoptosis, immune response, proliferation, or retinal degeneration related changes were noted in hESC-RPE, while important RPE specific proteins involved in key RPE functions such as visual cycle and phagocytosis, could be detected in the hESC-RPE. Overall, the results indicated that the proteome of the hESC-RPE cells closely resembled that of their native counterparts.

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