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Neuron. 2017 Jul 19;95(2):357-367.e4. doi: 10.1016/j.neuron.2017.06.039.

Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit.

Author information

1
RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902, Japan.
2
RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
3
RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; RIKEN BSI-KAO Collaboration Center, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902, Japan. Electronic address: hokto_kazama@brain.riken.jp.

Abstract

How cell-type-specific physiological properties shape neuronal functions in a circuit remains poorly understood. We addressed this issue in the Drosophila mushroom body (MB), a higher olfactory circuit, where neurons belonging to distinct glomeruli in the antennal lobe feed excitation to three types of intrinsic neurons, α/β, α'/β', and γ Kenyon cells (KCs). Two-photon optogenetics and intracellular recording revealed that whereas glomerular inputs add similarly in all KCs, spikes were generated most readily in α'/β' KCs. This cell type was also the most competent in recruiting GABAergic inhibition fed back by anterior paired lateral neuron, which responded to odors either locally within a lobe or globally across all lobes depending on the strength of stimuli. Notably, as predicted from these physiological properties, α'/β' KCs had the highest odor detection speed, sensitivity, and discriminability. This enhanced discrimination required proper GABAergic inhibition. These results link cell-type-specific mechanisms and functions in the MB circuit.

KEYWORDS:

Drosophila; cell-type specificity; electrophysiology; mushroom body; olfactory processing; synaptic integration; two-photon optogenetics

PMID:
28728024
DOI:
10.1016/j.neuron.2017.06.039
[Indexed for MEDLINE]
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