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J Tradit Complement Med. 2016 Nov 24;7(3):301-306. doi: 10.1016/j.jtcme.2016.10.001. eCollection 2017 Jul.

Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cells.

Author information

1
Department of Pharmacology, Faculty of Medicine, Islamic University of Malang, Indonesia.
2
Department of Internal Medicine, School of Medicine, University of Brawijaya, Indonesia.
3
Department of Biology, Faculty of Science, University of Brawijaya, Indonesia.
4
Department of Pharmacology, School of Medicine, University of Brawijaya, Indonesia.

Abstract

This study aims to determine the incretin effects of Urena lobata leaves extract on the structure and function of rats islet β-cells. This study utilizes male Sprague-Dawley rats divided into 2 control group and 3 test group (n = 5). Diabetic rats were induced with High Fructose Diet (HFD) and single dose intraperitoneal streptozotocin 25 mg/kg bw. Aqueous leaves extract of U. lobata was prepared by decoction methods and administrated orally with doses of 250, 500, and 1000 mg/kg bw for 4 weeks then incretin effect was evaluated by measuring serum GLP-1, insulin, and blood glucose levels. Histology of islet β-cells was evaluated using photomicroscopy by analyzing size, shape, and number. Data were analyzed using ANOVA test followed by LSD test and p ≤ 0.05 is considered significant. Oral administration of aqueous extract U. lobata leaves at doses of 250, 500, and 1000 mg/kg body weight were able to prolong GLP-1 bioavailability by 3-fold, 5-fold, and 7-fold respectively when compared to the diabetic group whereas blood glucose level were decreased about 30%, 35%, and 40% respectively (p < 0.05). Extract at doses of 500 and 1000 mg/kg bw also increased insulin level by 4-fold and 8-fold respectively compared to the diabetic group and the islet β-cells were repaired. The active compound in U. lobata leaves extract are suggested to prevent degradation of GLP-1 by inhibition of DPP-4 activity. Aqueous extract of U. lobata also improved the structure and function of islet β-cells by increasing of GLP-1 bioavailability.

KEYWORDS:

GLP-1; Incretin; Insulin; Islet β-cells; U. lobata

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