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J Mater Sci Mater Med. 2017 Aug;28(8):128. doi: 10.1007/s10856-017-5941-4. Epub 2017 Jul 18.

Reciprocal influence of hMSCs/HaCaT cultivated on electrospun scaffolds.

Author information

1
Department of Chemistry, University at Albany, State University of New York, Albany, NY, 12222, USA. sirsendu.tito@gmail.com.
2
Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069, Dresden, Germany. sirsendu.tito@gmail.com.
3
Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India. sirsendu.tito@gmail.com.
4
Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069, Dresden, Germany.
5
Biomaterials Department, INNOVENT e.V., Prüssingstraße 27B, 07745, Jena, Germany.
6
Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
7
Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069, Dresden, Germany. Dieter.Scharnweber@tu-dresden.de.

Abstract

Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α). Electrospun scaffold independent of GAGs alone did not affect the epithelial differentiation of hMSCs but combination of keratinocyte-hMSC contact co-culture and electrospun scaffold promotes the epithelial differentiation of hMSCs.

PMID:
28721664
DOI:
10.1007/s10856-017-5941-4
[Indexed for MEDLINE]

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