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Antonie Van Leeuwenhoek. 2017 Dec;110(12):1593-1611. doi: 10.1007/s10482-017-0910-y. Epub 2017 Jul 18.

The diversity and antibiotic properties of actinobacteria associated with endemic deepwater amphipods of Lake Baikal.

Author information

1
Institute of Biology, Irkutsk State University, Irkutsk, Russia.
2
Institute of Biology, Irkutsk State University, Irkutsk, Russia. denis.axengri@gmail.com.
3
Baikal Research Centre, Irkutsk, Russia. denis.axengri@gmail.com.
4
Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrucken, Germany.
5
Pharmazeutische Biotechnologie, Universit├Ąt des Saarlandes, Saarbrucken, Germany.
6
Baikal Research Centre, Irkutsk, Russia.

Abstract

The emergence of pathogenic bacteria resistant to antibiotics increases the need for discovery of new effective antimicrobials. Unique habitats such as marine deposits, wetlands and caves or unexplored biological communities are promising sources for the isolation of actinobacteria, which are among the major antibiotic producers. The present study aimed at examining cultivated actinobacteria strains associated with endemic Lake Baikal deepwater amphipods and estimating their antibiotic activity. We isolated 42 actinobacterial strains from crustaceans belonging to Ommatogammarus albinus and Ommatogammarus flavus. To our knowledge, this is the first report describing the isolation and initial characterization of representatives of Micromonospora and Pseudonocardia genera from Baikal deepwater invertebrates. Also, as expected, representatives of the genus Streptomyces were the dominant group among the isolated species. Some correlations could be observed between the number of actinobacterial isolates, the depth of sampling and the source of the strains. Nevertheless, >70% of isolated strains demonstrated antifungal activity. The dereplication analysis of extract of one of the isolated strains resulted in annotation of several known compounds that can help to explain the observed biological activities. The characteristics of ecological niche and lifestyle of deepwater amphipods suggests that the observed associations between crustaceans and isolated actinobacteria are not random and might represent long-term symbiotic interactions.

KEYWORDS:

Actinobacteria; Amphipods; Baikal; Endemics; Ommatogammarus spp.

PMID:
28721507
DOI:
10.1007/s10482-017-0910-y
[Indexed for MEDLINE]

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