Format

Send to

Choose Destination
Artif Cells Nanomed Biotechnol. 2018 Aug;46(5):994-1000. doi: 10.1080/21691401.2017.1354303. Epub 2017 Jul 18.

Antinociceptive synergistic interaction between Achillea millefolium and Origanum vulgare L. extract encapsulated in liposome in rat.

Author information

1
a Department of Anesthesiology , Sari University of Medical Sciences , Mazandaran , Iran.
2
b Department of Medical Biotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical sciences , Tehran , Iran.

Abstract

AIM:

This study aims to evaluate the antinociceptive effect of combined Achillea millefolium and Origanum extract encapsulated in liposome.

METHODS:

The effect of Achillea millefolium and Origanum vulgare L. extract, and their liposome-incorporated form was assessed using 3% formalin test in rat. 12 male Wistar rats, 4 in each group, were used in this study, and increasing doses of Achillea millefolium (31.6, 100, 178, and 316 mg/kg) and Origanum vulgare L. extract (5.6, 10, 17.8, and 31.6 mg/kg), and co-administered extract were i.p. administered 10 min before 3% formalin. The mechanisms of action were evaluated for the liposomal encapsulated co-administered extract using N(G)-nitro-l-arginine methyl ester (L-NAME) (3 mg/kg) and naloxone (1 mg/kg).

RESULTS:

The interaction index and isobolographic analysis revealed a synergistic effect of the extracts. We observed a lower experimental ED30 as compared to the theoretical ED30. Naloxone also reduced the antinociceptive effect of the liposome encapsulated co-administered extract.

CONCLUSION:

These data suggest that the Achillea millefolium and Origanum vulgare L. extract encapsulated in liposome gave a synergistic effect.

KEYWORDS:

Origanum vulgare L. extract; Achillea millefolium; antinociception; encapsulation; liposome

PMID:
28720004
DOI:
10.1080/21691401.2017.1354303
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center