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Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):113-118. doi: 10.14715/cmb/2017.63.5.21.

miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer.

Author information

1
Department of Breast Surgery , Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang 110042, China.
2
Department of General Surgery, the Fourth Affiliated Hospital of China Medical University, Shenyang 110000, China.
3
Department of General Surgery, The 463th Affiliated Hospital of the Chinese People's Liberation Army, Shenyang 110000, China.

Abstract

Breast cancer is the most commonly diagnosed cancer in women and a leading cause of cancer mortality. MicroRNAs (miRNAs) have been found to play a key role in proliferation, metastasis and invasion of cancer. In previous study, we found that miRNA-223 was significant expression inexosome derived from peripheral blood serum of breast cancer patients than in samples from control subjects, Therefor,the role ofmiRNA-223willbe researched in MCF-7 breast cancer cells.In this study, to explore the role of miRNA-223in influencing cell proliferation, metastasis and invasion of breast cancer, TargetScan tools (http://www.targetscan.org/vert_71/) was used to scan target genes of miRNA-223, and thenmiRNA expression, real time PCR, Western blotting andluciferase report assay were used to test regulates relationship of miRNA-223and its targets,cell viability and BrdU analysiswere used to test cell proliferation of MCF-7 breast cancer cells after expression miRNA-223inhibitor. Scanning targets of miRNA-223found FOXO1 was listed in targets content, and luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between FOXO1 and miRNA-223. Results showedthat miRNA-223inhibitorexpression increased protein expression level of FOXO1 in MCF-7 breast cancer cells,meanwhile, cell viability and BrdU analysis showed MCF-7 breast cancer cells were suppressed proliferation after up-regulation of FOXO1.In conclusion, we demonstrated that the miRNA-223can maintain cell proliferation of breast cancer cell through targeting FOXO 1, these results provide a new insight in tumor marker and potential therapeutic targets for breast cancer.

KEYWORDS:

Breast Cancer; Cell proliferation.; FOXO1; microRNA

PMID:
28719355
DOI:
10.14715/cmb/2017.63.5.21
[Indexed for MEDLINE]

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