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Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):113-118. doi: 10.14715/cmb/2017.63.5.21.

miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer.

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Department of Breast Surgery , Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang 110042, China.
Department of General Surgery, the Fourth Affiliated Hospital of China Medical University, Shenyang 110000, China.
Department of General Surgery, The 463th Affiliated Hospital of the Chinese People's Liberation Army, Shenyang 110000, China.


Breast cancer is the most commonly diagnosed cancer in women and a leading cause of cancer mortality. MicroRNAs (miRNAs) have been found to play a key role in proliferation, metastasis and invasion of cancer. In previous study, we found that miRNA-223 was significant expression inexosome derived from peripheral blood serum of breast cancer patients than in samples from control subjects, Therefor,the role ofmiRNA-223willbe researched in MCF-7 breast cancer cells.In this study, to explore the role of miRNA-223in influencing cell proliferation, metastasis and invasion of breast cancer, TargetScan tools ( was used to scan target genes of miRNA-223, and thenmiRNA expression, real time PCR, Western blotting andluciferase report assay were used to test regulates relationship of miRNA-223and its targets,cell viability and BrdU analysiswere used to test cell proliferation of MCF-7 breast cancer cells after expression miRNA-223inhibitor. Scanning targets of miRNA-223found FOXO1 was listed in targets content, and luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between FOXO1 and miRNA-223. Results showedthat miRNA-223inhibitorexpression increased protein expression level of FOXO1 in MCF-7 breast cancer cells,meanwhile, cell viability and BrdU analysis showed MCF-7 breast cancer cells were suppressed proliferation after up-regulation of FOXO1.In conclusion, we demonstrated that the miRNA-223can maintain cell proliferation of breast cancer cell through targeting FOXO 1, these results provide a new insight in tumor marker and potential therapeutic targets for breast cancer.


Breast Cancer; Cell proliferation.; FOXO1; microRNA

[Indexed for MEDLINE]

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