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Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):7-10. doi: 10.14715/cmb/2017.63.5.2.

miR-675 promotes disease progression of non-small cell lung cancer via activating NF-κB signaling pathway.

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Department of Pathology, The First Hospital of Wuhan, Wuhan, Hubei, China.
Department of Respiratory, The third people's hospital of Hubei Province, Wuhan, Hubei, China.
Department of hepatopathy of Hubei Zhongshan Hospital, Wuhan, Hubei, China.


Non-small cell lung cancer (NSCLC) is a major type of lung cancer significantly affected human life. Current studies have revealed potential role of miR-675 in the etiology of lung cancer. Therefore, in this study, we aimed to investigate the role of miR-675 in NSCLC cell line. We recruited 92 cases of NSCLC patients from July 2009 to February 2011 in the oncology department. Tumor and peripheral tissue was collected for miR-675 expression. NSCLC cell lines with either miR-675 over-expression or low-expression were established. Western blotting and immunofluorescence staining were used for detecting P65 and pP65 expression and translocation in the nuclei. MiR-675 expression was significantly higher in the lung cancer tissue than the peripheral normal tissue (P < 0.001). The gene expression of pP65 was also increased and decreased in cells with over-expression or low-expression of miR-675, respectively (both P < 0.05). Whereas the trend of P65 expression was on the opposite (both P < 0.05), indicating the NF-κB signaling pathway was activated when miR-675 was expressed and vice versa. Immunofluorescence staining showed that more pP65 expression was clustered in the nuclei in miR-675 over-expressed cells, which further demonstrated NF-κB signaling pathway activation. Increased miR-675 expression is associated with NSCLC progression through activation of NF-κB signaling pathway.


NF-κB; Non-small cell lung cancer.; miR-675

[Indexed for MEDLINE]

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