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Mar Drugs. 2017 Jul 18;15(7). pii: E228. doi: 10.3390/md15070228.

A Transcriptomic Survey of Ion Channel-Based Conotoxins in the Chinese Tubular Cone Snail (Conus betulinus).

Huang Y1,2, Peng C3, Yi Y4,5, Gao B6, Shi Q7,8,9.

Author information

1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. huangyu@genomics.cn.
2
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. huangyu@genomics.cn.
3
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. pengchao@genomics.cn.
4
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. yiyunhai@genomics.cn.
5
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. yiyunhai@genomics.cn.
6
Hainan Provincial Key Laboratory of Research and Development of Tropical Medicinal Plants, Hainan Medical University, Haikou 571199, China. gaobingmiao1982@163.com.
7
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. shiqiong@genomics.cn.
8
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China. shiqiong@genomics.cn.
9
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. shiqiong@genomics.cn.

Abstract

Conotoxins in the venom of cone snails (Conus spp.) are a mixture of active peptides that work as blockers, agonists, antagonists, or inactivators of various ion channels. Recently we reported a high-throughput method to identify 215 conotoxin transcripts from the Chinese tubular cone snail, C. betulinus. Here, based on the previous datasets of four transcriptomes from three venom ducts and one venom bulb, we explored ion channel-based conotoxins and predicted their related ion channel receptors. Homologous analysis was also performed for the most abundant ion channel protein, voltage-gated potassium (Kv; with Kv1.1 as the representative), and the most studied ion channel receptor, nicotinic acetylcholine receptor (nAChR; with α2-nAChR as the representative), in different animals. Our transcriptomic survey demonstrated that ion channel-based conotoxins and related ion channel proteins/receptors transcribe differentially between the venom duct and the venom bulb. In addition, we observed that putative κ-conotoxins were the most common conotoxins with the highest transcription levels in the examined C. betulinus. Furthermore, Kv1.1 and α2-nAChR were conserved in their functional domains of deduced protein sequences, suggesting similar effects of conotoxins via the ion channels in various species, including human beings. In a word, our present work suggests a high-throughput way to develop conotoxins as potential drugs for treatment of ion channel-associated human diseases.

KEYWORDS:

Chinese tubular cone snail; conotoxin; ion channel receptor; transcriptome

PMID:
28718820
PMCID:
PMC5532670
DOI:
10.3390/md15070228
[Indexed for MEDLINE]
Free PMC Article

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