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J Agric Food Chem. 2017 Sep 6;65(35):7624-7628. doi: 10.1021/acs.jafc.7b02255. Epub 2017 Jul 28.

Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.

Author information

1
Department of Bioscience and Biotechnology, Fukui Prefectural University , 4-1-1 Matsuoka-Kenjojima, Eiheiji, Fukui 910-1195, Japan.
2
Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology , Nagoya, Aichi 466-8555, Japan.
3
Clinical Pathology, Osaka Medical College , 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
4
Graduate School of Environmental and Human Sciences, Faculty of Pharmacy, Meijo University , Tempaku, Nagoya, Aichi 468-8503, Japan.
5
Analytical and Measuring Instruments Division, Shimadzu Corporation , 1 Kuwabara, Kyoto 604-8511, Japan.

Abstract

Plant hormones act as important signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Because their concentrations of hormones control the physiological responses in the target tissue, it is important to know the distributions and concentrations in the tissues. However, it is difficult to determine the hormone concentration on the plant tissue as a result of the limitations of conventional methods. Here, we report the first multi-imaging of two plant hormones, one of cytokinin [i.e., trans-zeatin (tZ)] and abscisic acid (ABA) using a new technology, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) imaging. Protonated signals of tZ (m/z 220.1) and ABA (m/z 265.3) were chosen on longitudinal sections of rice roots for MS imaging. tZ was broadly distributed about 40 mm behind the root apex but was barely detectable at the apex, whereas ABA was mainly detected at the root apex. Multi-imaging using MALDI-TOF-MS enabled the visualization of the localization and quantification of plant hormones. Thus, this tool is applicable to a wide range of plant species growing under various environmental conditions.

KEYWORDS:

abscisic acid (ABA); cytokinin; imaging mass spectrometry (IMS); matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI−TOF−MS); trans-zeatin (tZ)

PMID:
28718648
DOI:
10.1021/acs.jafc.7b02255
[Indexed for MEDLINE]

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