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Genes Dev. 2017 Jun 1;31(11):1073-1088. doi: 10.1101/gad.298232.117.

From structure to mechanism-understanding initiation of DNA replication.

Author information

1
DNA Replication Group, Institute of Clinical Sciences (ICS), Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom.
2
Medical Research Council (MRC) London Institute of Medical Sciences (LMS), London W12 0NN, United Kingdom.

Abstract

DNA replication results in the doubling of the genome prior to cell division. This process requires the assembly of 50 or more protein factors into a replication fork. Here, we review recent structural and biochemical insights that start to explain how specific proteins recognize DNA replication origins, load the replicative helicase on DNA, unwind DNA, synthesize new DNA strands, and reassemble chromatin. We focus on the minichromosome maintenance (MCM2-7) proteins, which form the core of the eukaryotic replication fork, as this complex undergoes major structural rearrangements in order to engage with DNA, regulate its DNA-unwinding activity, and maintain genome stability.

KEYWORDS:

CMG; DNA replication; MCM2–7; cryo-EM; pre-RC; replisome

PMID:
28717046
PMCID:
PMC5538431
DOI:
10.1101/gad.298232.117
[Indexed for MEDLINE]
Free PMC Article

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