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Mol Cell Biol. 2017 Sep 12;37(19). pii: e00225-17. doi: 10.1128/MCB.00225-17. Print 2017 Oct 1.

Cooperative Repression of Insulin-Like Growth Factor Type 2 Receptor Translation by MicroRNA 195 and RNA-Binding Protein CUGBP1.

Zhang Y1,2, Zhang Y1,2, Xiao L1,2, Yu TX1,2, Li JZ1,2, Rao JN1,2, Turner DJ1,2, Gorospe M3, Wang JY4,2,5.

Author information

1
Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.
2
Baltimore Veterans Affairs Medical Center, Baltimore, Maryland, USA.
3
Laboratory of Genetics and Genomics, National Institute on Aging-IRP, NIH, Baltimore, Maryland, USA.
4
Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA jywang@som.umaryland.edu.
5
Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Abstract

Insulin-like growth factor type 2 (IGF2) receptor (IGF2R) recognizes mannose 6-phosphate-containing molecules and IGF2 and plays an important role in many pathophysiological processes, including gut mucosal adaptation. However, the mechanisms that control cellular IGF2R abundance are poorly known. MicroRNAs (miRNAs) and RNA-binding proteins (RBPs) critically regulate gene expression programs in mammalian cells by modulating the stability and translation of target mRNAs. Here we report that miRNA 195 (miR-195) and RBP CUG-binding protein 1 (CUGBP1) jointly regulate IGF2R expression at the posttranscriptional level in intestinal epithelial cells. Both miR-195 and CUGBP1 interacted with the 3' untranslated region (3'-UTR) of Igf2r mRNA, and the association of CUGBP1 with Igf2r mRNA enhanced miR-195 binding to Igf2r mRNA. Ectopically expressed CUGBP1 and miR-195 repressed IGF2R translation cooperatively without altering the stability of Igf2r mRNA. Importantly, the miR-195- and CUGBP1-repressed levels of cellular IGF2R led to a disruption in the structure of the trans-Golgi network. These findings indicate that IGF2R expression is controlled posttranscriptionally by two factors that associate with Igf2r mRNA and suggest that miR-195 and CUGBP1 dampen IGF signaling by inhibiting IGF2R translation.

KEYWORDS:

RNA-binding proteins; insulin superfamily; intestinal epithelial cells; noncoding RNAs; posttranscriptional regulation; trans-Golgi network

PMID:
28716948
PMCID:
PMC5599715
DOI:
10.1128/MCB.00225-17
[Indexed for MEDLINE]
Free PMC Article

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