Rapamycin inhibits oxidative/nitrosative stress and enhances angiogenesis in high glucose-treated human umbilical vein endothelial cells: Role of autophagy

Biomed Pharmacother. 2017 Sep:93:885-894. doi: 10.1016/j.biopha.2017.07.044. Epub 2017 Jul 13.

Abstract

Chronic hyperglycemia is a potent risk factor of abnormal angiogenesis with various tissue diseases. Autophagy, as an alternative cell response, is mostly generated by a vast array of insults. Applying autophagic response contributes to normal cell retrieval circumstance during various insults. We aimed to show whether stimulation/inhibition of autophagy could reduce or exacerbate oxidative status and angiogenic potential in endothelial cells after exposure to 30mM glucose. HUVECs were incubated with the combined regime of 100nM Rapamycin and 30mM glucose over a period of 72h. The effect of rapamycin on cell viability, malondialdehyde levels, and nitric oxide were monitored by convenient assays. Intracellular ROS level was measured by flow cytometric analysis and DCFDA. HUVECs migration and angiogenic properties were assessed using scratch test and tubulogenesis assay. The expression of autophagic modulators LC3, Becline-1 and P62 was measured by using western blotting. Data showed 30mM glucose reduced cell viability, migration and in vitro tubulogenesis and level of ROS and nitric oxide were found to increased (p<0.05). Rapamycin had potential to increase cell survival and significantly decreased the total levels of oxidative stress markers after cell exposure to 30mM glucose (p<0.05). Rapamycin potentially improved the detrimental effect of 30mM glucose on cell migration and tubulogenesis capacity (p<0.05). Effective autophagic response was stimulated by rapamycin by increasing beclin-1, and the LC3-II/I ratio and reducing intracellular P62 level (p<0.05), resulting in the improvement of cell health and function. Together, rapamycin protected HUVECs from damages caused by high glucose concentration. This effect was possibly mediated by autophagy-dependent pathway.

Keywords: 2′,7′-Dichlorofluorescin diacetate (PubChem CID: 77718); 3-Methyladenine; 3-Methyladenine (PubChem CID: 1673); Autophagy; Diabetes; Dimethyl Sulfoxide (PubChem CID: 679); Human umbilical vein endothelial cells; Monodansylcadaverine (PubChem CID: 4247); Oxidative stress; Phosphoric acid (PubChem CID: 1004); Rapamycin; Rapamycin (PubChem CID: 5284616); Thiazolyl blue tetrazolium bromide (PubChem CID: 64965); Thiobarbituric acid (PubChem CID: 2723628); d-Glucose (PubChem CID: 5793).

MeSH terms

  • Autophagy / drug effects*
  • Beclin-1 / metabolism
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Glucose / pharmacology*
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • Neovascularization, Pathologic / drug therapy*
  • Neovascularization, Pathologic / metabolism
  • Nitric Oxide / metabolism
  • Nitrosative Stress / drug effects*
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species / metabolism
  • Sirolimus / pharmacology*

Substances

  • Beclin-1
  • Reactive Oxygen Species
  • Nitric Oxide
  • Glucose
  • Sirolimus