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J Pharm Biomed Anal. 2017 Oct 25;145:379-385. doi: 10.1016/j.jpba.2017.06.029. Epub 2017 Jul 5.

New approach for the diagnosis of histamine intolerance based on the determination of histamine and methylhistamine in urine.

Author information

1
Department of Nutrition, Food Sciences and Gastronomy, XaRTA, INSA, School of Pharmacy and Food Sciences, University of Barcelona, Avinguda Prat de la Riba 171, 08921, Santa Coloma de Gramenet, Spain.
2
Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, Via del Castro Laurenziano 9, 00161, Rome, Italy.
3
Department of Nutrition, Food Sciences and Gastronomy, XaRTA, INSA, School of Pharmacy and Food Sciences, University of Barcelona, Avinguda Prat de la Riba 171, 08921, Santa Coloma de Gramenet, Spain. Electronic address: mcvidal@ub.edu.

Abstract

Histamine intolerance is a disorder in the homeostasis of histamine due to a reduced intestinal degradation of this amine, mainly caused by diamine oxidase (DAO) enzyme deficiency, which provokes its accumulation in plasma and the appearance of adverse health affects. A new approach for the diagnosis of this intolerance could be the determination of histamine and its metabolites in urine. The aim of this work was to develop and validate a rapid method to determine histamine and methylhistamine in human urine by Ultra High Performance Liquid Chromatography and Fluorimetric detection (UHPLC-FL). The proposed method is a consistent procedure to determine histamine and methylhistamine in less than 11min with adequate linearity and sensitivity. Relative standard deviation was always lower than 5.5%, ensuring method precision; and mean recovery was greater than 99% for both analytes. The structure of histamine and methylhistamine conjugated with OPA were confirmed by UHPLC-ITD-FTMS which enabled to unequivocally identify both analytes in standards and also in urine samples. The analysis of histamine and methylhistamine in urine samples could be a potential new approach for the routine diagnosis of histamine intolerance, more patient-friendly and with clear advantages in terms of equipment and personnel demand for sample collection in comparison with current plasmatic DAO activity determination.

KEYWORDS:

Diamine oxidase; Histamine; Histamine intolerance; Methylhistamine; Solid phase extraction; Ultra high pressure liquid chromatography

PMID:
28715791
DOI:
10.1016/j.jpba.2017.06.029
[Indexed for MEDLINE]

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