Format

Send to

Choose Destination
J Hazard Mater. 2017 Oct 15;340:231-240. doi: 10.1016/j.jhazmat.2017.06.058. Epub 2017 Jun 27.

PFOA-induced metabolism disturbance and multi-generational reproductive toxicity in Oryzias latipes.

Author information

1
Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research (NIER), Incheon 404-708, Republic of Korea.
2
Department of Chemistry, Pusan National University, Busan 46241, Republic of Korea.
3
Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research (NIER), Incheon 404-708, Republic of Korea. Electronic address: mirrorpark@korea.kr.

Abstract

The aims of this study were to examine multi-generational reproductive toxicity and metabolism disturbances in Oryzias latipes exposed to 0.3, 3, and 30mg/L PFOA for 259-day. The highest concentration of PFOA suppressed fecundity over three generations from F0 to F2 and sac-fry survival rate in F2 generation, indicating that PFOA resulted in multi-generational reproductive toxicity (p<0.05). Histologically, in F1 and F2 generations, O. latipes exposed to 30mg/L PFOA revealed accelerated gonad development, and the atrophy and degeneration of thyroid follicular cell. Glucose content showed the highest increase in both genders in all metabolites. However, alanine, glutamine, threonine, and lactate content, which are converted into glucose showed decline tendency, suggesting that PFOA led to gluconeogenesis. Change of osmolyte content affecting osmosis such as a decrease of male myo-inositol (m-Ino), an increase of female trimethylamine N-oxide (TMAO) and an increase of male dimethylamine (DMA) suggest that PFOA might affect osmoregulation of O. latipes. Oxaloacetate of male fish and succinate of female fish showed significant alterations, indicating that PFOA may affect energy metabolism differently by sex. These findings will help elucidate the toxicity of PFOA in diverse biological responses including metabolism change.

KEYWORDS:

Metabolism disturbance; Multi-generational reproductive toxicity; Oryzias latipes; Perfluorooctanoic acid

PMID:
28715746
DOI:
10.1016/j.jhazmat.2017.06.058
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center