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J Vis Exp. 2017 Jul 6;(125). doi: 10.3791/55952.

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer.

Author information

1
Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden.
2
Department of General, Gastrointestinal and Transplant Surgery, University of Heidelberg.
3
Department of Pathology, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden.
4
Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden; German Cancer Consortium (DKTK); German Cancer Research Center (DKFZ).
5
Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden; German Cancer Consortium (DKTK); German Cancer Research Center (DKFZ); Sebastian.Schoelch@uniklinikum-dresden.de.

Abstract

Despite the advantages of easy applicability and cost-effectiveness, colorectal cancer mouse models based on tumor cell injection have severe limitations and do not accurately simulate tumor biology and tumor cell dissemination. Genetically engineered mouse models have been introduced to overcome these limitations; however, such models are technically demanding, especially in large organs such as the colon in which only a single tumor is desired. As a result, an immunocompetent, genetically engineered mouse model of colorectal cancer was developed which develops highly uniform tumors and can be used for tumor biology studies as well as therapeutic trials. Tumor development is initiated by surgical, segmental infection of the distal colon with adeno-cre virus in compound conditionally mutant mice. The tumors can be easily detected and monitored via colonoscopy. We here describe the surgical technique of segmental adeno-cre infection of the colon, the surveillance of the tumor via high-resolution colonoscopy and present the resulting colorectal tumors.

PMID:
28715385
PMCID:
PMC5609309
[Available on 2019-07-06]
DOI:
10.3791/55952
[Indexed for MEDLINE]

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