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Front Cell Infect Microbiol. 2017 Jun 30;7:300. doi: 10.3389/fcimb.2017.00300. eCollection 2017.

Streptococcus pyogenes Phospholipase A2 Induces the Expression of Adhesion Molecules on Human Umbilical Vein Endothelial Cells and Aorta of Mice.

Author information

1
Division of Microbiology and Infectious Diseases, Graduate School of Medical and Dental Sciences, Niigata UniversityNiigata, Japan.
2
Department of Microbiology and Infection Control Sciences, Kyoto Pharmaceutical UniversityKyoto, Japan.
3
Research Center for Advanced Oral Science, Graduate School of Medical and Dental Sciences, Niigata UniversityNiigata, Japan.
4
Department of Oral and Molecular Microbiology, Osaka University, Graduate School of DentistryOsaka, Japan.

Abstract

The Streptococcus pyogenes phospholipase A2 (SlaA) gene is highly conserved in the M3 serotype of group A S. pyogenes, which often involves hypervirulent clones. However, the role of SlaA in S. pyogenes pathogenesis is unclear. Herein, we report that SlaA induces the expression of intercellular adhesion molecule 1 (ICAM1) and vascular cell adhesion molecule 1 (VCAM1) via the arachidonic acid signaling cascade. Notably, recombinant SlaA induced ICAM1 and VCAM1 expression in human umbilical vein endothelial cells (HUVECs), resulting in enhanced adhesion of human monocytic leukemia (THP-1) cells. However, C134A, a variant enzyme with no enzymatic activity, did not induce such events. In addition, culture supernatants from S. pyogenes SSI-1 enhanced the adhesion of THP-1 cells to HUVECs, but culture supernatants from the ΔslaA isogenic mutant strain had limited effects. Aspirin, a cyclooxygenase 2 inhibitor, prevented the adhesion of THP-1 cells to HUVECs and did not induce ICAM1 and VCAM1 expression in HUVECs treated with SlaA. However, zileuton, a 5-lipoxygenase inhibitor, did not exhibit such effects. Furthermore, pre-administration of aspirin in mice intravenously injected with SlaA attenuated the transcriptional abundance of ICAM1 and VCAM1 in the aorta. These results suggested that SlaA from S. pyogenes stimulates the expression of adhesion molecules in vascular endothelial cells. Thus, SlaA contributes to the inflammation of vascular endothelial cells upon S. pyogenes infection.

KEYWORDS:

HUVEC; ICAM1; S. pyogenes; SlaA; VCAM1; phospholipase A2

PMID:
28713783
PMCID:
PMC5491884
DOI:
10.3389/fcimb.2017.00300
[Indexed for MEDLINE]
Free PMC Article

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