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J Biol Chem. 2017 Sep 29;292(39):16368-16379. doi: 10.1074/jbc.M117.780528. Epub 2017 Jul 14.

A pH-dependent switch promotes β-synuclein fibril formation via glutamate residues.

Author information

1
From the Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey 08854.
2
From the Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey 08854 sagar.khare@rutgers.edu.
3
From the Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey 08854 jean.baum@rutgers.edu.

Abstract

α-Synuclein (αS) is the primary protein associated with Parkinson's disease, and it undergoes aggregation from its intrinsically disordered monomeric form to a cross-β fibrillar form. The closely related homolog β-synuclein (βS) is essentially fibril-resistant under cytoplasmic physiological conditions. Toxic gain-of-function by βS has been linked to dysfunction, but the aggregation behavior of βS under altered pH is not well-understood. In this work, we compare fibril formation of αS and βS at pH 7.3 and mildly acidic pH 5.8, and we demonstrate that pH serves as an on/off switch for βS fibrillation. Using αS/βS domain-swapped chimera constructs and single residue substitutions in βS, we localized the switch to acidic residues in the N-terminal and non-amyloid component domains of βS. Computational models of βS fibril structures indicate that key glutamate residues (Glu-31 and Glu-61) in these domains may be sites of pH-sensitive interactions, and variants E31A and E61A show dramatically altered pH sensitivity for fibril formation supporting the importance of these charged side chains in fibril formation of βS. Our results demonstrate that relatively small changes in pH, which occur frequently in the cytoplasm and in secretory pathways, may induce the formation of βS fibrils and suggest a complex role for βS in synuclein cellular homeostasis and Parkinson's disease.

KEYWORDS:

Parkinson disease; beta-synuclein; computer modeling; fibril; neurodegeneration; nuclear magnetic resonance (NMR); protein aggregation; synuclein

PMID:
28710275
PMCID:
PMC5625065
DOI:
10.1074/jbc.M117.780528
[Indexed for MEDLINE]
Free PMC Article

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