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J AOAC Int. 2017 Nov 1;100(6):1607-1623. doi: 10.5740/jaoacint.17-0207. Epub 2017 Jul 13.

Identification of Microorganisms by Modern Analytical Techniques.

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Nicolaus Copernicus University, Faculty of Chemistry, Department of Environmental Chemistry and Bioanalytics, Gagarina 7, 87-100 Torun, Poland; Nicolaus Copernicus University, Interdisciplinary Centre of Modern Technology, Wileńska 4, 87-100 Torun, Poland.


Rapid detection and identification of microorganisms is a challenging and important aspect in a wide range of fields, from medical to industrial, affecting human lives. Unfortunately, classical methods of microorganism identification are based on time-consuming and labor-intensive approaches. Screening techniques require the rapid and cheap grouping of bacterial isolates; however, modern bioanalytics demand comprehensive bacterial studies at a molecular level. Modern approaches for the rapid identification of bacteria use molecular techniques, such as 16S ribosomal RNA gene sequencing based on polymerase chain reaction or electromigration, especially capillary zone electrophoresis and capillary isoelectric focusing. However, there are still several challenges with the analysis of microbial complexes using electromigration technology, such as uncontrolled aggregation and/or adhesion to the capillary surface. Thus, an approach using capillary electrophoresis of microbial aggregates with UV and matrix-assisted laser desorption ionization time-of-flight MS detection is presented.

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