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PLoS One. 2017 Jul 12;12(7):e0180452. doi: 10.1371/journal.pone.0180452. eCollection 2017.

In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy.

Author information

1
Osaka Bioscience Institute, Suita, Osaka, Japan.
2
Medical Innovation Center, Kyoto University Graduate School of Medicine, Kyoto, Japan.
3
Frontier Research Center for Post-genome Science and Technology, Hokkaido University, Sapporo, Japan.

Abstract

A combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capability, and therefore its axial resolution is generally poor. At present, it is unclear whether wide-field microscopy can visualize activity of densely packed small neurons at cellular resolution. To examine the applicability of wide-field microscopy for small-sized neurons, we recorded calcium activity of dentate granule cells having a small soma diameter of approximately 10 micrometers. Using a combination of high numerical aperture (0.8) objective lens and independent component analysis-based image segmentation technique, activity of putative single granule cell activity was separated from wide-field calcium imaging data. The result encourages wider application of wide-field microscopy in in vivo neurophysiology.

PMID:
28700611
PMCID:
PMC5507494
DOI:
10.1371/journal.pone.0180452
[Indexed for MEDLINE]
Free PMC Article

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