Format

Send to

Choose Destination
Acta Cir Bras. 2017 Jun;32(6):440-448. doi: 10.1590/s0102-865020170060000004.

Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats.

Author information

1
MSc, Postgraduate Program in Surgical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Conception and design of the study; surgical procedures; acquisition, data, analysis and interpretation of data; statistical analysis, manuscript preparation and writing; critical revision; final approval.
2
PhD, Division of Coloproctology, Hospital de Clínicas de Porto Alegre, UFRGS, Porto Alegre-RS, Brazil. Analysis and interpretation of data, statistical analysis, manuscript writing, critical revision, final approval.
3
MD, Postgraduate Program in Surgical Sciences, UFRGS, Porto Alegre-RS, Brazil. Surgical procedures, acquisition of data, statistical analysis, critical revision, final approval.
4
Graduate student, Medical School, UFRGS, Porto Alegre-RS, Brazil. Surgical procedures, acquisition of data.
5
Associate Professor, PhD, Division of General Surgery, Department of Surgery, Hospital de Clínicas de Porto Alegre, UFRGS, Porto Alegre-RS, Brazil. Conception and design of the study, analysis and interpretation of data, manuscript preparation and writing, critical revision, final approval.

Abstract

Purpose: :

To investigate if fluorescein fluorescent test can predict dehiscence in a model of ischemic colonic anastomosis in rats.

Methods: :

This experimental controlled trial randomly assigned 55 rats to four groups. Anastomoses were performed in non-ischemic colon segments (control group) and in ischemic colon segments measuring 1, 2 or 3 cm long (groups 1, 2 and 3, respectively). Fluorescein was injected and the tissues were examined under ultraviolet light. Seven days later, a second-look surgery was performed to check for the presence or absence of anastomosis dehiscence.

Results: :

Twenty-four rats presented anastomotic dehiscence during the second-look surgery. Reticular and nonfluorescent patterns were significantly associated with the occurrence of anastomotic dehiscence. Fluorescein fluorescence had a sensitivity of 95.8%, specificity of 89.2%, positive predictive value of 88.4%, negative predictive value of 96.2%, and accuracy of 92.3% to predict anastomotic dehiscence.

Conclusion: :

Fluorescein fluorescent test can accurately predict leak in a model of ischemic colonic anastomosis in rats.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Scientific Electronic Library Online
Loading ...
Support Center