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Br J Haematol. 2017 Aug;178(4):603-615. doi: 10.1111/bjh.14691. Epub 2017 Jul 12.

Telomere length and somatic mutations in correlation with response to immunosuppressive treatment in aplastic anaemia.

Author information

1
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
2
Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
3
Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

We investigated the frequencies of cytogenetic aberrations and somatic mutations of prognostic relevance in 393 patients with aplastic anaemia (AA). Clonality was determined by G-banding/fluorescence in situ hybridization (FISH) (n = 245), and targeted capture sequencing was performed for 88 haematopoiesis-related genes (n = 70). The telomere length (TL) of bone marrow nucleated cells was measured at the single cell level by FISH (n = 135). Eighteen (4·6%) patients showed disease progression, and monosomy 7 (50·0%) was the most predominant cytogenetic evolution at disease transformation. One third of patients (32·9%) presented at least 1 mutation; the most frequently mutated genes were NOTCH1, NF1, SCRIB, BCOR and DNMT3A. The patient group with clonal changes (30·7%) showed an adverse response to immunosuppressive treatment (IST), compared to the non-clonal group, but this finding did not show statistical significance. The TL of AA patients was significantly shorter than normal control and patients with clonal changes showed significantly shorter TLs. Patients with TL>5·9 showed a higher response rate to IST (P = 0·048). In conclusion, the patients with clonal changes or TL attrition showed a poor response to IST. Shorter TL can be used not only as a biomarker, but also as a predictive marker for treatment response to IST.

KEYWORDS:

aplastic anaemia; immunosuppressive treatment; somatic mutations; telomere length

PMID:
28699658
DOI:
10.1111/bjh.14691
[Indexed for MEDLINE]

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