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3 Biotech. 2017 Aug;7(4):238. doi: 10.1007/s13205-017-0861-z. Epub 2017 Jul 11.

Zinc oxide-curcumin nanocomposite loaded collagen membrane as an effective material against methicillin-resistant coagulase-negative Staphylococci.

Author information

1
School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India.
2
International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India.
3
Sree Narayana Institute of Medical Sciences, Chalakka, Ernakulam, Kerala, 683 594, India.
4
School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India. radhakrishnanek@mgu.ac.in.

Abstract

Zinc oxide nanoparticles and curcumin are excellent antimicrobial agents. They have the potential to be used as alternative to antibiotics in wound infection management. In this study, ZnO-curcumin nanocomposite was synthesized and characterized. Physical adsorption of the nanocomposite onto collagen skin wound dressing was conducted and structural investigation was carried out by SEM. Antimicrobial assay, minimum inhibitory concentration (MIC), and viability assays of different concentrations of nanocomposite loaded collagen membrane were conducted against clinically isolated methicillin-resistant coagulase-negative Staphylococci (MRCoNS), such as S. epidermidis, S. hemolyticus, and S. saprophyticus. The nanocomposite showed excellent anti-CoNS activity on time kill assay with the MIC value of 195 µg/mL against S. epidermidis, S. hemolyticus and 390 µg/mL against S. saprophyticus. The nanocomposite loaded collagen membrane also showed excellent in vitro antistaphylococcal activity. This study may lead to the development of antibiotic alternate strategies to control and limit the MRCoNS in wound-related infections.

KEYWORDS:

Antistaphylococcal; Curcumin; Methicillin-resistant coagulase-negative Staphylococci; Nanocomposite; Zinc oxide

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