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Gene. 2017 Sep 10;628:1-7. doi: 10.1016/j.gene.2017.07.019. Epub 2017 Jul 8.

Galleria mellonella is low cost and suitable surrogate host for studying virulence of human pathogenic Vibrio cholerae.

Author information

1
Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan. Electronic address: habib@comsats.edu.pk.
2
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), H-12, Islamabad 44000, Pakistan.
3
Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan.
4
Welcome Trust, Sanger Institute, Cambridge, UK; Department of Pathogen Molecular Biology, London School of Hygiene & Tropical Medicine, UK.
5
Department of Pathogen Molecular Biology, London School of Hygiene & Tropical Medicine, UK. Electronic address: Brendan.Wren@lshtm.ac.uk.

Abstract

Vibrio cholerae causes a severe diarrheal disease affecting millions of people worldwide, particularly in low income countries. V. cholerae successfully persist in aquatic environment and its pathogenic strains results in sever enteric disease in humans. This dual life style contributes towards its better survival and persistence inside host gut and in the environment. Alternative animal replacement models are of great value in studying host-pathogen interaction and for quick screening of various pathogenic strains. One such model is Galleria mellonella, a wax moth which has a complex innate immune system and here we investigate its suitability as a model for clinical human isolates of O1 El TOR, Ogawa serotype belonging to two genetically distinct subclades found in Pakistan (PSC-1 and PSC-2). We demonstrate that the PSC-2 strain D59 frequently isolated from inland areas, was more virulent than PSC-1 strain K7 mainly isolated from coastal areas (p=0.0001). In addition, we compared the relative biofilm capability of the representative strains as indicators of their survival and persistence in the environment and K7 showed enhanced biofilm forming capabilities (p=0.004). Finally we present the annotated genomes of the strains D59 and K7, and compared them with the reference strain N16961.

KEYWORDS:

Galleria mellonella; Surrogate host model; V. cholerae; Virulence studies

PMID:
28698162
DOI:
10.1016/j.gene.2017.07.019
[Indexed for MEDLINE]
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