Format

Send to

Choose Destination
Int J Biol Macromol. 2017 Dec;105(Pt 1):204-212. doi: 10.1016/j.ijbiomac.2017.07.026. Epub 2017 Jul 8.

In vitro immunological and anti-complementary activities of two water-soluble lignins from Zizyphus jujube cv. Jinchangzao.

Author information

1
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar, 843300, China; Engineering Laboratory of Chemical Resources Utilization in South Xinjiang of Xinjiang Production & Construction Corps, Tarim University, Alar, 843300, China.
2
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar, 843300, China.
3
College of Chemistry & Pharmacy, Northwest A & F University, Yangling, 712100, Shaanxi, China.
4
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar, 843300, China; Engineering Laboratory of Chemical Resources Utilization in South Xinjiang of Xinjiang Production & Construction Corps, Tarim University, Alar, 843300, China. Electronic address: skybhj@taru.edu.cn.

Abstract

Two homogenous biological macromolecules, designated as JJC1 and JJC2 were extracted from Zizyphus jujube cv. Jinchangzao. Their molecular weights were determined to be 56.03 and 112.11kDa by high performance gel permeation chromatography (HPGPC), respectively. Chemical and spectral analysis indicated that both JJC1 and JJC2 mainly consisted of lignin, along with carbohydrates (∼18%). Both JJC1 and JJC2 could stimulate the proliferation of spleen lymphocytes, and enhance phagocytosis and NO production of RAW264.7 cells. In addition, JJC2, but not JJC1 elicited an inhibitory effect on complement activation through the classical pathway (CH50: 2.73mg/mL) as well as the alternative pathway (AP50: 2.99mg/mL). These findings implied that water-soluble lignins were one of the bioactive components in Z. jujube, and further provided insights into the understanding of molecular basis for diverse medicinal and nutritional values of this fruit.

KEYWORDS:

Anti-complementary activity; Immunological activity; jujube lignin

PMID:
28698081
DOI:
10.1016/j.ijbiomac.2017.07.026
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center