Dynamical regimes of lipids in additivated liposomes with enhanced elasticity: A field-cycling NMR relaxometry approach

Biophys Chem. 2017 Sep:228:38-46. doi: 10.1016/j.bpc.2017.06.007. Epub 2017 Jun 27.

Abstract

We study the molecular dynamics of lipids in binary large unilamellar liposomes suspended in D2O composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) or soy phosphatidylcholine (SPC) additivated with different percentiles of sodium deoxycholate (SDC). We use the fast field-cycling proton NMR relaxometry technique over a wide timescale and at diverse temperatures. A model previously validated in different formulations is here employed for the relaxometric analysis of elastic vesicles. A new dynamical regime is observed for the first time in additivated DMPC and additivated/non-additivated SPC liposomes. This surprising feature is discussed in terms of vesicle shape fluctuations, enhanced elasticity and lipid & additive diffusion within the membrane. The continuum elastic theory is revisited for a better understanding of recent experiments and those here presented. We address the point of deformability measurements across rigid permeable barriers versus measurements of the bending elastic modulus in free-standing vesicles.

Keywords: Deformability; Lipid dynamics; Liposomes; Membrane flexibility; NMR relaxometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxycholic Acid / chemistry
  • Dimyristoylphosphatidylcholine / chemistry
  • Elasticity
  • Liposomes / chemistry*
  • Magnetic Resonance Spectroscopy*
  • Phosphatidylcholines / chemistry

Substances

  • Liposomes
  • Phosphatidylcholines
  • Deoxycholic Acid
  • Dimyristoylphosphatidylcholine