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Plant Cell. 2017 Jul;29(7):1642-1656. doi: 10.1105/tpc.17.00016. Epub 2017 Jul 10.

ZHOUPI and KERBEROS Mediate Embryo/Endosperm Separation by Promoting the Formation of an Extracuticular Sheath at the Embryo Surface.

Author information

1
Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.
2
Centre National de la Recherche Scientifique/University of Bordeaux, Plant Imaging Platform of Bordeaux Imaging Center, UMS 3420, F-33000 Bordeaux, France.
3
Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR5200, F-33000 Bordeaux, France.
4
Centre National de la Recherche Scientifique, Laboratoire de Biogenèse Membranaire, UMR5200, F-33000 Bordeaux, France.
5
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280.
6
Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280.
7
Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France gwyneth.ingram@ens-lyon.fr.

Abstract

Arabidopsis thaliana seed development requires the concomitant development of two zygotic compartments, the embryo and the endosperm. Following fertilization, the endosperm expands and the embryo grows invasively through the endosperm, which breaks down. Here, we describe a structure we refer to as the embryo sheath that forms on the surface of the embryo as it starts to elongate. The sheath is deposited outside the embryonic cuticle and incorporates endosperm-derived material rich in extensin-like molecules. Sheath production is dependent upon the activity of ZHOUPI, an endosperm-specific transcription factor necessary for endosperm degradation, embryo growth, embryo-endosperm separation, and normal embryo cuticle formation. We show that the peptide KERBEROS, whose expression is ZHOUPI dependent, is necessary both for the formation of a normal embryo sheath and for embryo-endosperm separation. Finally, we show that the receptor-like kinases GSO1 and GSO2 are required for sheath deposition at the embryo surface but not for production of sheath material in the endosperm. We present a model in which sheath formation depends on the coordinated production of material in the endosperm and signaling within the embryo, highlighting the complex molecular interaction between these two tissues during early seed development.

PMID:
28696222
PMCID:
PMC5559742
DOI:
10.1105/tpc.17.00016
[Indexed for MEDLINE]
Free PMC Article

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