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Sci Adv. 2017 Jul 5;3(7):e1700523. doi: 10.1126/sciadv.1700523. eCollection 2017 Jul.

A tail of two voltages: Proteomic comparison of the three electric organs of the electric eel.

Author information

1
Department of Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA.
2
Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA.
3
Department of Molecular and Cellular Medicine, Texas A&M University, College Station, TX 77843, USA.
4
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

Abstract

The electric eel (Electrophorus electricus) is unusual among electric fishes because it has three pairs of electric organs that serve multiple biological functions: For navigation and communication, it emits continuous pulses of weak electric discharge (<1 V), but for predation and defense, it intermittently emits lethal strong electric discharges (10 to 600 V). We hypothesized that these two electrogenic outputs have different energetic demands reflected by differences in their proteome and phosphoproteome. We report the use of isotope-assisted quantitative mass spectrometry to test this hypothesis. We observed novel phosphorylation sites in sodium transporters and identified a potassium channel with unique differences in protein concentration among the electric organs. In addition, we found transcription factors and protein kinases that show differential abundance in the strong versus weak electric organs. Our findings support the hypothesis that proteomic differences among electric organs underlie differences in energetic needs, reflecting a trade-off between generating weak voltages continuously and strong voltages intermittently.

KEYWORDS:

Electrophorus electricus; electric eel; quantitative proteomics and phosphoproteomics

PMID:
28695212
PMCID:
PMC5498108
DOI:
10.1126/sciadv.1700523
[Indexed for MEDLINE]
Free PMC Article

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