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Sci Rep. 2017 Jul 10;7(1):4967. doi: 10.1038/s41598-017-04785-4.

Expression and regulation of drug transporters in vertebrate neutrophils.

Author information

1
The Bateson Centre, The University of Sheffield, Sheffield, S10 2TN, UK.
2
Department of Chemistry, The University of Sheffield, Sheffield, S3 7HF, UK.
3
Department of Infection, Immunity & Cardiovascular Disease, The University of Sheffield, Sheffield, S10 2RX, UK.
4
Institute of Biology, University of Leiden, 2333 CC, Leiden, The Netherlands.
5
Immuno-Inflammation Therapy Area Unit, GlaxoSmithKline Research and Development Ltd., Stevenage, SG1 2NY, UK.
6
Rare Diseases Research Unit, Pfizer Ltd., Cambridge, CB21 6GP, UK.
7
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 3EG, UK.
8
Department of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, CB2 2XY, UK.
9
The Bateson Centre, The University of Sheffield, Sheffield, S10 2TN, UK. s.a.renshaw@sheffield.ac.uk.
10
Department of Infection, Immunity & Cardiovascular Disease, The University of Sheffield, Sheffield, S10 2RX, UK. s.a.renshaw@sheffield.ac.uk.

Abstract

There remains a need to identify novel pro-resolution drugs for treatment of inflammatory disease. To date, there are no neutrophil-specific anti-inflammatory treatments in clinical use, perhaps due to our lack of understanding of how drugs access this complex cell type. Here we present the first comprehensive description and expression of both major classes of drug transporters, SLC and ABC, in resting human blood neutrophils. Moreover, we have studied the expression of these carriers in the tractable model system, the zebrafish (Danio rerio), additionally examining the evolutionary relationship between drug transporters in zebrafish and humans. We anticipate that this will be a valuable resource to the field of inflammation biology and will be an important asset in future anti-inflammatory drug design.

PMID:
28694436
PMCID:
PMC5504015
DOI:
10.1038/s41598-017-04785-4
[Indexed for MEDLINE]
Free PMC Article

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