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Development. 2017 Aug 1;144(15):2737-2747. doi: 10.1242/dev.144089. Epub 2017 Jul 10.

BMP signaling regulates satellite cell-dependent postnatal muscle growth.

Author information

1
Versailles Saint-Quentin-en-Yvelines University, INSERM U1179, LIA BAHN CSM, Montigny-le-Bretonneux 78180, France.
2
Department of Neuropediatrics and NeuroCure Clinical Research Center, Charité University-Medicine, Berlin 10117, Germany.
3
Pierre et Marie Curie University, Paris Sorbonne, INSERM, UMRS974, CNRS FRE3617, Center for Research in Myology, Paris 75013, France.
4
Developmental Biology/Signal Transduction Group, Max Delbruck Center for Molecular Medicine, Berlin 13092, Germany.
5
Department of Cardiac Development and Remodeling, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim 61231, Germany.
6
Department of Cardiovascular Physiology, Ruhr University Bochum, D-44780 Bochum, Germany.
7
INSERM, Paris Est University, IMRB U955-E10, Créteil 94010, France.
8
Paul Sabatier University, Inserm UMR 1048, Toulouse 31432, France.
9
SQY Therapeutics, Montigny-le-Bretonneux 78180, France.
10
Paris Descartes-Sorbonne Paris Cité University, Inserm UMR1163, Imagine Institute, Paris 75015, France.
11
Versailles Saint-Quentin-en-Yvelines University, INSERM U1179, LIA BAHN CSM, Montigny-le-Bretonneux 78180, France helge.amthor@uvsq.fr.
12
Pediatric Department, University Hospital Raymond Poincaré, Garches 92380, France.

Abstract

Postnatal growth of skeletal muscle largely depends on the expansion and differentiation of resident stem cells, the so-called satellite cells. Here, we demonstrate that postnatal satellite cells express components of the bone morphogenetic protein (BMP) signaling machinery. Overexpression of noggin in postnatal mice (to antagonize BMP ligands), satellite cell-specific knockout of Alk3 (the gene encoding the BMP transmembrane receptor) or overexpression of inhibitory SMAD6 decreased satellite cell proliferation and accretion during myofiber growth, and ultimately retarded muscle growth. Moreover, reduced BMP signaling diminished the adult satellite cell pool. Abrogation of BMP signaling in satellite cell-derived primary myoblasts strongly diminished cell proliferation and upregulated the expression of cell cycle inhibitors p21 and p57 In conclusion, these results show that BMP signaling defines postnatal muscle development by regulating satellite cell-dependent myofiber growth and the generation of the adult muscle stem cell pool.

KEYWORDS:

BMP signaling; Mouse; Muscle stem cells; Postnatal muscle growth; Satellite cells

PMID:
28694257
PMCID:
PMC5560039
DOI:
10.1242/dev.144089
[Indexed for MEDLINE]
Free PMC Article

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