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J Vet Sci. 2018 Jan 31;19(1):59-70. doi: 10.4142/jvs.2018.19.1.59.

Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae.

Author information

1
Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.

Abstract

Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than that of wild-type (WT) B. henselae. To study the differences in proteomic expression between WT and Δomp43, two-dimensional gel electrophoresis-based proteomic analysis was performed. Based on Clusters of Orthologus Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and 3 proteins associated with cellular processing and signaling. By semi-quantitative reverse transcriptase polymerase chain reaction, increases in tldD, efp, ntrX, pdhA, purB, and ATPA mRNA expression and decreases in Rho and yfeA mRNA expression were confirmed in Δomp43. In conclusion, this is the first report showing that a loss of OMP43 expression in B. henselae leads to retarded proliferation. Furthermore, our proteomic data provide useful information for the further investigation of mechanisms related to the growth of B. henselae.

KEYWORDS:

Bartonella; omp43; outer membrane protein; proliferation; proteomics

PMID:
28693313
PMCID:
PMC5799401
DOI:
10.4142/jvs.2018.19.1.59
[Indexed for MEDLINE]
Free PMC Article

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